F127/Cisplatin Microemulsions: In Vitro, In Vivo and Computational Studies

The development of effective strategies for local administration chemotherapeutic drugs, thus minimizing the adverse side effects to patients, is one key challenges in biomedicine and cancer research. This work reports formulation characterization PluronicF127 microemulsions enhance bioavailability Cisplatin (Cis). size Cis microemulsion was about 12.0 nm, as assessed by dynamic light scattering analysis. In vitro cytotoxic activity free F127/Cis were studied on malignant (C152 MCF7) normal (HUVEC) cells via tetrazolium (MTT) colorimetric assay. Cell morphology also monitored. assessments revealed thatF127/Cis induced cytotoxicity/morphological changes a lesser extent than Cis. Regarding vivo experiments, injected intraperitoneally at 7 14 mg/kg doses into adult male Wistar rats assess histologic biochemical changes. this case, bulk group caused severe histopathological significant increases serum liver enzymes kidney function markers. treated with dose showed fatty enzymes, blood urea nitrogen, creatinine levels. low nano-Cis increase levels accompanied mild liver. Theoretical surveys performed get an understanding interplay between F127 Results reveal that hydrogen bonding (HB) interactions F127have influence molecular properties may playa role lower toxicity comparison